(1) The extent of lysosomal lysis in the hypotonic solution, i.e. the degree of their sensitivity to the osmotic shock, can be examined by the measurement of their enzyme latency loss.(2) However, at closer examination the other organelles are certainly mentioned, as they are implicated in lysosomal , peroxisomal, or mitochondrial diseases.(3) The leukocytes eventually destroy the pathogens by phagocytosis and lysosomal fusion that expose bacteria to lytic enzymes.(4) Intracellular bacteria are subjected to several bacteriocidal mechanisms in the macrophages such as free oxygen radicals and phagosome lysosomal activity.(5) Furthermore, it is not enough simply to treat patients with recombinant enzymes - modifications must be made so that the infused enzyme is targeted to lysosomes in relevant cells and tissues.(6) For instance, within the individual cell, the subcellular sites of photo-damage may include the plasma membrane, the lysosomes and the mitochondria.(7) These efforts have successfully identified mitochondria, lysosomes and various cytoplasmic membranes as among the primary targets of PDT-mediated damage, depending on the particular photosensitizer.(8) In all eukaryotic cells the lysosomes or vacuoles play major cellular turnover roles, including autophagy where entire organelles are delivered to them for turnover.(9) The osmotic gradient across the membranes can drive an influx of water, which causes the lysosomal lysis.(10) The spindle cell population consisted of bipolar cells with large oval nuclei that contained macronucleoli and scattered rough endoplasmic reticulum, mitochondria, and lysosomes within their cytoplasm.
